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A single-cell suspension from the selected tissue(s) is embedded in low melting point agarose, lysed, and electrophoresed under alkaline (pH > 13) conditions. The assay measures DNA damage (i.e., strand breaks, DNA adducts, excision repair sites, and cross-links) at the single-cell level. The test protocol. The Assay Protocol described below is written as a Quick Reference using alkaline. Comet Control Cells (cat# CC) for two well slides. Reagents and.

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The comet assay or single-cell gel (SCG) test is a microgel electrophoresis technique that measures DNA damage at the level of single cells. A small number of. A single-cell suspension from the selected tissue(s) is embedded in low melting point agarose, lysed, and electrophoresed under alkaline (pH > 13) conditions. Comet assay utilizes single cells to measure DNA damage. First, cells are embedded into agarose and then placed onto a slide. The slide is then immersed into.

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A single-cell suspension from the selected tissue(s) is embedded in low melting point agarose, lysed, and electrophoresed under alkaline (pH > 13) conditions. The comet assay or single-cell gel (SCG) test is a microgel electrophoresis technique that measures DNA damage at the level of single cells. A small number of. Alkaline single cell gel electrophoresis in A cells. Detecting DNA damage in A cells using the Comet Assay. AUTHORED BY: control protocols.